Reconstruction differences rival individual differences in the fly compass connectome and break trained circuit models

Md Ali Ashraf Yad, Nazifa Tabassum Nabila

Neural network models are increasingly constructed from synapse-resolution connectomes, and the same circuit is now available in reconstructions from other animals and pipelines. We do not know whether such models are dependent on the reconstruction they are built from, and whether connectome differences are biological or measurement errors. We studied the Drosophila head-direction circuit in nine versions: four flies (male CNS, hemibrain, Fly Wire, BANC) and five variants of the same fly varying only by thresholds, proofreading or synapse detection. Using wiring-based correspondences validated by anatomical labels, we place all differences on one scale. A change of synapse detection within one fly changed the circuit more than halfway to the smallest inter-fly difference on four distances; proofreading differences were smaller but depended on the metric, and left and right halves of one fly differed no more than a random half. Networks trained on each wiring to hold a cued heading developed near-perfect ring attractors, but their error increased from below 1 degree to 10-28 degrees when all weights changed by 1%. Training on each wiring did not transfer to any other connectome,…